Figure 4 | Scientific Reports

Figure 4

From: Isolation and characterization of myogenic precursor cells from human cremaster muscle

Figure 4

In vitro isolation, expansion and differentiation of human cremaster muscle-derived myogenic precursor cells. (A) Schematic representation of the male reproductive system anatomy, showing cremaster muscle (red) and the biopsy sample zone is highlighted by a rectangle. Outline of the myosphere suspension culture and myotube differentiation (adhesion culture) steps. (B,C) Optical microscope images of the suspension culture showing cells and tissue fragments (arrow) at day 0 (B) and spheres at day 7 (C). (DF) Optical microscope images of the adhesion culture at day 2 (D) and day 9 (E,F), where multinucleated myotubes (arrows) become predominant. Scale bars, 100 μm.