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Figure 3

From: Pitx2 cholinergic interneurons are the source of C bouton synapses on brainstem motor neurons

Figure 3

Verification of C bouton identity via postsynaptic m2 immunoreactivity. In white anti-m2 antibody, in green anti-ChAT antibody labels motor neuron somata and cholinergic C boutons on them, in red endogenous tdTomato+ fluorescence from Pitx2 derived synapses. (A) Pitx2 derived (red) C boutons (green) on the accessory nucleus (green) in alignment with m2 (white). (B) Dorsal nucleus of vagus lacks m2 immunoreactivity and C boutons. (C) Pitx2 derived (red) C boutons (green) on the hypoglossal nucleus (green) in alignment with m2 (white). (D) Pitx2 derived (red) C boutons (green) on the nucleus ambiguus (green) in alignment with m2 (white). (E) Pitx2 derived (red) C boutons (green) on the facial nucleus (green) in alignment with m2 (white). (F) Nuclus abducens lacks m2 immunoreactivity and C boutons. (G) Pitx2 derived (red) C boutons (green) on the trigeminal nucleus (green) in alignment with m2 (white). (H) Trochlear/oculomotor nucleus lacks m2 immunoreactivity and C boutons. Scale bar: 2 μm.

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