Figure 2 | Scientific Reports

Figure 2

From: PARP1 is a versatile factor in the regulation of mRNA stability and decay

Figure 2

Our experimental design to determine the effect of PARP1 and PARylation on mRNA transcription and stability. Both WT and KD cells were treated overnight with PJ34 to inhibit PARylation (Stage 1). Next, cells were treated with DRB to inhibit transcription (Stage 2). After three hours DRB treatment, cells were washed and transcription was allowed to recover in fresh media without DRB. Cells were then collected at the specified times post DRB-wash (Stage 3).