Table 3 Single editing site contribution to changes in editing patterns of different 5-Ht2cr pre-mRNA isoforms between PWScrp/m5LoxP and wild type mice choroid plexus samples.

From: Ectopic expression of Snord115 in choroid plexus interferes with editing but not splicing of 5-Ht2c receptor pre-mRNA in mice

Edited Site Mean RPKM WT Mean RPKM LoxP Fold change (WT/LoxP) P-value, SIG WT, % LoxP, %
1 2275 2225 1 0.67, no 0.2 0.2
2 4129 3295 1.3 0.14, no 0.4 0.4
3 2119 2078 1 0.8, no 0.2 0.2
A 148907 100311 1.5 0.000057**** 14.7 11.3
B 66074 37479 1.8 0.0003*** 6.5 4.2
C 234736 190987 1.2 0.0004*** 23.2 21.6
D 904161 782103 1.2 0.006** 89.5 88.4
E 51944 45946 1.1 0.15, no 5.1 5.2
F 1877 1891 1 0.94, no 0.2 0.2
G 3242 2378 1.4 0.14, no 0.3 0.23
H 1940 1878 1 0.84, no 0.2 0.26
  1. Editing site: The editing sites are labeled according to Fig. 1A. Mean RPKM WT and LoxP: Calculated mean of all RPKM values for 5-Ht2cr mRNA editing patterns accounting for site contribution in wild type (WT) and, PWScrp/m5LoxP (LoxP) editing. Fold change WT/LoxP: Fold change between wild type (WT) and PWScrp/m5LoxP (LoxP) editing sites. P-value: P-value of Student’s t-test. SIG: Graphpad formatting - asterisks describe p-values levels of statistical significance. False Discovery Rate (pBH) values were calculated for the correction of multiple testing; pBH values 0.0006, 0.001, 0.0016 and 0.016 for editing sites A, B, C and D, respectively, confirmed significances of the t-test. WT(%) and LoxP(%): The percentage of detected editing in the depicted sites in wild type (WT) and PWScrp/m5′LoxP (LoxP) mouse choroid plexuses is indicated. Percentage is calculated based on the mean RPKM values for 5-Ht2cr mRNA-edited isoforms in investigated genotypes.