Figure 7 | Scientific Reports

Figure 7

From: Effectiveness of ZnPc and of an amine derivative to inactivate Glioblastoma cells by Photodynamic Therapy: an in vitro comparative study

Figure 7

Apoptosis induction: Annexin V/PI staining. T98G cells were incubated with TAZnPc or ZnPc dissolved in DMEM supplemented with 4% FBS plus antibiotics at a concentration of 0.5 µM during 18 hours. Then, medium was replaced by fresh medium not containing Pcs and cells were irradiated using two light doses: 10 J/cm2 or 27 J/cm2. Three hours after irradiation, floating and attached cells were collected and washed. Then cells were stained using Annexin V conjugated to FITC (staining used to determine exposed phosphatidylserine) and PI and analyzed by flow cytometry. A representative dot plot graph in which cells in the lower left quadrant are considered living cells (Annexin V−PI−), cells in the upper left quadrant are early apoptotic cells (Annexin V+PI−), cells in the upper right quadrant are late apoptotic cells (Annexin V+PI+) and cells in the lower right quadrant are necrotic cells (Annexin V− PI+). The mean percentage ± SEM of cells in each quadrant with respect to the total number of cells is represented below in the bar graph. The result is a representative experiment of three performed in triplicate. Individual plots are presented in Supplementary Fig. 6.