Figure 4 | Scientific Reports

Figure 4

From: Effectiveness of ZnPc and of an amine derivative to inactivate Glioblastoma cells by Photodynamic Therapy: an in vitro comparative study

Figure 4

Effect of the photosensitizers on T98G cell viability. T98G cells were incubated in the presence of 0.125 (light gray), 0.25 (dark gray) or 0.5 µM (white) of: (a) TAZnPc and (b) ZnPc dissolved in DMEM supplemented with 4% FBS plus antibiotics during 18 hours. Then the medium was replaced with fresh medium without Pcs and the cells irradiated using two different light doses: 10 or 27 J/cm2. Cell viability was measured 24 hours after PDT using alamarBlue as described under Materials and Methods. Results are the mean ± SEM of cell viability with respect to the control (non-irradiated cells in absence of Pc) of three independent experiments performed in triplicate. ***p < 0.005 and ****p < 0.001 with respect to the control (non irradiated cells in the absence of Pc) using two-way ANOVA with Dunett’s post-test.