Figure 3 | Scientific Reports

Figure 3

From: Effectiveness of ZnPc and of an amine derivative to inactivate Glioblastoma cells by Photodynamic Therapy: an in vitro comparative study

Figure 3

Subcellular localization of TAZnPc and ZnPc in T98G cells (a) Subcellular localization of Pcs. Cells were incubated with Pcs at 0.5 μM for 18 hours, then, where incubated with organelles specific probes (Lysosome upper row and Mitochondria lower row) and observed in vivo under a confocal microscope. Pcs fluorescence (column 1 and 4), organelle-specific probe fluorescence (column 2 and 5), and merged images (column 3 and 6) are presented. (b) Fluorescence intensity profile. In order to confirm the identity of the observed intracellular fluorescence, we excited with a laser at 635 nm and recorded the fluorescence at different wavelength. (c) Pearson’s coefficient between Pc signal and the organelle-specific probe signal was calculated using Image J.

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