Figure 10 | Scientific Reports

Figure 10

From: Effectiveness of ZnPc and of an amine derivative to inactivate Glioblastoma cells by Photodynamic Therapy: an in vitro comparative study

Figure 10

Effect of the photosensitizers on glioma cell viability. Three glioma cell lines (MO59, LN229 and U87-MG) were incubated in the presence of 0.125 (light gray), 0.25 (dark gray) or 0.5 µM (white) of: (a) TAZnPc or (b) ZnPc dissolved in DMEM supplemented with 4% FBS plus antibiotics during 18 hours. Then the medium was replaced with fresh medium without Pc and the cells irradiated using two different light doses: 10 or 27 J/cm2. Cell viability was measured 24 hours after PDT using alamarBlue as described under Materials and Methods. Results are the mean ± SEM of three independent experiments performed in triplicate. *p < 0.05 **p < 0.01 ***p < 0.005 and ****p < 0.001 with respect to the control (non irradiated cells in the absence of Pc) using two-way ANOVA with Dunnett’s post-test.