Table 1 Ribosome binding site strengths of original and designed operons.

From: Genetically engineered biosynthetic pathways for nonnatural C60 carotenoids using C5-elongases and C50-cyclases in Escherichia coli

Organism Gene RBS strength in v1 plasmid Designed RBS strength in v2 plasmid
M. luteus crtE2 812 4296
crtYg 0.01 1525
crtYh 140 4700
Dietzia sp. CQ4 lbtA 968 4106
lbtBC 15 4700
C. glutamicum crtYe 4493 3891
crtYf 4342 5886
crtEb 389 5066
C. efficiens crtYe 776 1113
crtYf 33 2663
crtEb 93 1990
  1. RBS strength obtained using a ribosome binding site (RBS) calculator18,19,20. The RBS and open reading frame (ORF) sequences are listed in Supplementary Tables S1 and S2.