PrP octa-repeat domain does not alter expression in HeLa cells. (A) Reporter construct bearing the complete PrP octa-repeat domain sandwiched bewtween mCherry and EYFP. The construct with two fluorescent proteins only served as control. (B) Flow cytometry analysis of HeLa cells transfected with the reporter construct with or without PrP octa-repeat domain and treated with 0, 2.5 and 10 µM PDS (n = 2). (C) Representative immunoblot (n = 5) of full-length fusion protein detected by anti-mCherry antibody. NPT2 served as transfection control.