Figure 4 | Scientific Reports

Figure 4

From: Human renal fibroblasts are strong immunomobilizers during a urinary tract infection mediated by uropathogenic Escherichia coli

Figure 4

UPEC invasion of renal fibroblasts. Immunofluorescence staining of CFT073 following stimulation of renal fibroblasts for 3 hours. Staining of the fibroblasts cell nuclei was performed with DAPI and is shown in blue. (C,D) CFT073 are stained (B) in red (extracellular) prior to permeabilization, and (A) in green (extracellular and intracellular) after permeabilization. Merged image of (A,B) where intracellular bacteria are seen as green stain (arrows) and extracellular bacteria are shown as merged red and green (yellow) stain (arrows). (C,D) Stained CFT073 and cell nuclei is merged with bright field image of renal fibroblasts. (D) Representative images from three independent experiments are shown. Scale bar: 50 µm. Invasion was evaluated following stimulated with CFT073, CFT073Δpap and CFT073ΔfimH for 3 hours. (E) Invasion was presented as percentage of CFT073 invasion. Data are presented as mean ± SEM (n = 3 independent experiments). Asterisks denote statistical significance compared to CFT073 (*p < 0.05).