Figure 2 | Scientific Reports

Figure 2

From: Human renal fibroblasts are strong immunomobilizers during a urinary tract infection mediated by uropathogenic Escherichia coli

Figure 2

IL-8 release from renal fibroblasts. Primary human renal fibroblasts were stimulated with UTI89, MG1655, CFT073, CFT073Δpap, CFT073ΔfimH, CFT073ΔhlyA, CFT073ΔTcpC and CFT073ΔfliC at MOI 10 (A), adenosine triphosphate (100 µM, ATP), Adenosine (100 µM), lipopolysaccharide (1 µg/ml, LPS), flagellin (1 µg/ml) and Pam3CSK4 (1 µg/ml) for 6 hours. (C) The fibroblasts were also pre-incubated with DMSO (vehicle), p38 inhibitor SB203580 (10 µM), ERK1/2 inhibitor PD98059 (10 µM), NF-κB inhibitor BAY 11-7082 (5 µM) and the PKC inhibitor bisindolylmaleimide I (10 µM) for 1 hours prior to CFT073 stimulation for 6 hours at MOI 10. (B) IL-8 release was analyzed after stimulation. Data are presented as mean ± SEM (n = 4 independent experiments). Asterisks denote statistical significance compared to respective unstimulated control cells (*p < 0.05, ***p < 0.001).