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aAgonist is the compound used to generate concentration-response curve.
bModulator is the compound used in defined concentrations.
cpKA are values estimated for the agonist.
dpKB are values estimated for the modulator.
Data are means ± SEM, n = 4.
Flp-In T-REx 293 cells harbouring a human GPR84-Gαi2 fusion protein were treated with doxycycline (100 ng.ml−1, 24 h) to induced expression of the fusion protein. [35S]GTPγS binding studies were then performed as described in Fig. 1 and binding affinity and cooperativity factors extracted from such data.