The effects of activators of GPR84 in RAW264.7 cells are on-target. Membranes were generated from LPS-treated RAW264.7 cells (a,b). These were used in [35S]GTPγS binding studies in which the effect of varying concentrations of compound 107 was used to block effects of EC80 concentrations of either 2-HTP (a) or PSB-16671 (b). The ability of compound 107 to block effects of either 2-HTP (c) or PSB-16671 (d) to promote binding of [35S]GTPγS to either human or mouse GPR84-Gαi2 fusion proteins expressed in Flp-In T-REx 293 cells is also displayed. Data represent means ± S.E.M. of combined data from experiments performed on 7 (a) or 5 (b–d) individual membrane preparations.