Figure 7 | Scientific Reports

Figure 7

From: Prenatal Betamethasone interferes with immune system development and alters target cells in autoimmune diabetes

Figure 7

Betamethasone exposure alters gene expression in NIT-1 cells, islets of Langerhans and whole pancreas. (A) Relative gene expression of 13 selected genes in NIT-1 cells after betamethasone treatment (0.1 μM betamethasone, n ≥ 8) analysed by qRT-PCR. Gene expression was normalized to Gapdh. Bars show the mean ± s.d. of the Log2 of Fold Change (FC) using basal (complete medium-cultured NIT-1) transcription as standard value (+p ≤ 0.05 and ++p < 0.01, Wilcoxon test). (B) Relative gene expression of 13 selected genes in purified islets of Langerhans from 4 mice after culture with betamethasone (0.1 μM betamethasone, n = 4) analysed by qRT-PCR. Gene expression was normalized to Gapdh. Bars show the mean ± s.d. of the Log2 of FC using basal (complete medium-cultured islets) transcription as standard value (Wilcoxon test); n.d., not detected. (C) Histogram of relative gene expression of 8 selected genes in pancreases from newborn pups prenatally treated with 0.1 mg betamethasone (grey bars) or vehicle control (black bars) (n = 4 pancreases/group) analysed by qRT-PCR. Gene expression was normalized to Gapdh. Bars show the mean ± s.d. of gene expression (Mann-Whitney test).

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