Figure 5 | Scientific Reports

Figure 5

From: Prenatal Betamethasone interferes with immune system development and alters target cells in autoimmune diabetes

Figure 5

Immune cell subsets in the spleen are altered after betamethasone treatment. (A) Weight and total number of cells in the spleen. White symbols represent the PBS group and grey symbols represent the betamethasone (bet) group, circles or dots and squares were used for postnatal day 1 (d1) and postnatal day 4 (d4), respectively. Bars and symbols show the mean ± s.d. of n ≥ 5 mice (*p ≤ 0.05, **p < 0.01, ***p < 0.001, Mann-Whitney test). (B) Percentage of neutrophils (Ly6G+CD11b+), macrophages (CD11cCD11b+), conventional dendritic cells (CD11c+CD11b+) and plasmacytoid dendritic cells (CD11c+CD11b) from the CD45+ CD3 population in the spleen. White symbols represent the sham group and grey symbols represent the bet group; circles or dots and squares were used for d1 and d4, respectively. Bars and symbols show the mean ± s.d. of n ≥ 5 mice (*p ≤ 0.05, **p < 0.01, Mann-Whitney test). (C) Percentage of viable lymphocytes (CD19+ B cells and TCRαβ+ T cells) and viable CD3 CD4+. Percentages of CD4+ cells, CD8+ cells and DN cells from the T cell population are only available at d4. White symbols represent the sham group and grey symbols represent the bet group; circles or dots and squares were used for d1 and d4, respectively. Bars and symbols show the mean ± s.d. of n ≥ 5 mice (*p ≤ 0.05, **p < 0.01, Mann-Whitney test). (D) t-SNE representation of the cell abundance (density) for each spleen lymphoid population analysed by flow cytometry at d1 and d4 after birth in control and bet groups. Asterisk (*) refers to a population that is probably lymphoid tissue inducer cells (LTIs), but no positive marker in the cytometry panel was used. The plots show two representative animals per group. (E) t-SNE representation of the cell abundance (density) for each spleen myeloid population analysed by flow cytometry at d1 and d4 after birth in control and bet groups. The plots show two representative animals per group.

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