Figure 4 | Scientific Reports

Figure 4

From: Prenatal Betamethasone interferes with immune system development and alters target cells in autoimmune diabetes

Figure 4

Prenatal betamethasone alters thymocyte maturation in the thymus. (A) Weight and total number of cells in the thymus. White symbols represent the control group and grey symbols represent the betamethasone (bet) group, circles or dots and squares were used for postnatal day 1 (d1) and postnatal day 4 (d4) after birth, respectively. Bars and symbols show the mean ± s.d. of n ≥ 5 mice (*p ≤ 0.05, **p < 0.01, ***p < 0.001, Mann-Whitney test). (B) Percentage of maturing thymocytes in the double negative (DN) stage (CD4 CD8), DN stage 1 (DN1, CD44+ CD25), DN stage 2 (DN2, CD44+ CD25+), DN stage 4 (DN4, CD44 CD25) from the DN population, CD8 immature single positive (CD8ISP, CD3 CD8+) and double positive (DP) stage (CD4+ CD8+). White symbols represent the sham group and grey symbols represent the bet group; circles or dots and squares were used for d1 and d4, respectively. Bars and symbols show the mean ± s.d. of n ≥ 5 mice (*p ≤ 0.05, **p < 0.01, Mann-Whitney test). (C) Percentage of viable CD4+ thymocytes, CD8+ thymocytes and γδ+ thymocytes. White symbols represent the sham group and grey symbols represent the bet group; circles or dots and squares were used for d1 and d4, respectively. Bars and symbols show the mean ± s.d. of n ≥ 5 mice (*p ≤ 0.05, **p < 0.01, Mann-Whitney test). (D) t-SNE representation of the cell abundance (density) for each thymic population analysed by flow cytometry at d1 and d4 after birth in control and bet groups. The plots show two representative animals per group.

Back to article page