Dendritic cells (DCs) derived from bone marrow precursors in the presence of betamethasone show a semi-mature phenotype after LPS stimuli. (A) Upper panel: percentage of viability of DCs (annexinV PE−, 7aad− of CD11chi). Lower panel: differentiation yield of DCs from bone marrow progenitors (% CD11c+). (B) Median of fluorescence intensity (MFI) of MHC class I, MHC class II, CD40, CD86 and CD25 surface expression on DCs (CD11c+). White circles represent immature DCs (iDCs) after differentiation. Black and grey symbols represent DCs stimulated with lipopolysaccharide (LPS) for 24 h, without betamethasone (bet) (mDCs, black squares), or with 10 nM bet (grey triangles), 100 nM bet (grey dots), 1000 nM bet (grey rhombus). Lines show the mean of 9 independent experiments (*p ≤ 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001, Dunn’s test, Friedman test).