Figure 4 | Scientific Reports

Figure 4

From: Erythrocytes as bioreactors to decrease excess ammonium concentration in blood

Figure 4

The calculated kinetic parameters of a quasi-stationary bioreactor containing GDH and AAT. The calculations were performed for a constant concentration of AMM in plasma equal to 0.5 mM. (a) Effect of the ratio of AAT/GDH activities on the rate of ammonium consumption (VAMM). GDH activity was constant and equal to 0.4 IU/mlRBCs, while AAT activity varied in the range of 0.04–4 IU/mlRBCs. (b) Dependence of VAMM on concentrations of encapsulated enzymes (at ААТ/GDH = const = 5) at different RBC membrane permeability for pyruvate (the permeability was increased compared to physiological by 1-, 2-, 4-, 8- and 16-fold for curves 1, 2, 3, 4 and 5, respectively). (c) Dependence of VAMM on changes in the permeability of the bioreactor membrane for pyruvate (1) or lactate (2) (for each curve, all other permeabilities are considered equal to physiological, GDH activity is 2048 IU/mlRBCs, and AAT/GDH activity ratio is 5). (d) Increase in alanine (ALA) concentration during quasi-stationary operation of a bioreactor containing GDH and AAT.

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