Figure 3 | Scientific Reports

Figure 3

From: Transthyretin Aggregation Pathway toward the Formation of Distinct Cytotoxic Oligomers

Figure 3

Increases in cytotoxicity of native TTR, and WT and G53A oligomers assessed using SH-SY5Y cells with LIVE/DEAD cell viability assay (Molecular Devices, CA). The mammalian cells were treated with native WT TTR and the oligomers at different concentrations (monomer concentration) for 48 hours, and the number of live and dead cells was analyzed. The oligomers were isolated from TTR samples (1 mg/ml) incubated at 4 °C for one week (Fig. S4).