Table 5 List of the GD resistant peptides identified by untargeted HR-MS/MS analysis and assessed to encrypt full length T-cell epitopes (*information retrieved by http://www.allergenonline.org/celiacbrowse.shtml66, **core epitope nomenclature by Sollid et al.68).

From: Scouting for Naturally Low-Toxicity Wheat Genotypes by a Multidisciplinary Approach

Sample Peptides matching full length T-cell epitopes (100% sequence identity) Known T-cell stimulatory epitopes
Experimental m/z [Da] Sequence Protein n°* Sequence (ID)* HLA-DQ molecule* 9 AA Restricted epitope**
REF 4 1075,022 (+2) QQPFPQTQQPQQPFPQQP γ-gliadin 3 QQPQQPFPQ (472) DQ2.5/DQ8 DQ2.5-glia-γ4c/DQ8-glia-γ1a
      PFPQTQQPQQPFPQ (553) DQ8 (DQ2/8) DQ2.5-glia-γ4c/DQ8-glia-γ1a
      PQTQQPQQPFPQ (926) DQ2 DQ2.5-glia-γ4c/DQ8-glia-γ1a
  635,660 (+3) QPFPQQPQQPFPLQPQ ω-gliadin 2 PFPQQPQQPF (538) DQ2 (Glia-γ2 (p89-p102))
      FPQQPQQPF (542) DQ2 (p90-p102)
  1409,676 (+1) SQQPQQPFPQPQ γ-gliadin 1 QQPQQPFPQ (472) DQ2.5/DQ8 DQ2.5-glia-γ4c/DQ8-glia-γ1a
  1419,705 (+1) PQQPQQPFPQPQ γ-gliadin/ω-gliadin 1 QQPQQPFPQ (472) DQ2.5/DQ8 DQ2.5-glia-γ4c/DQ8-glia-γ1a
  661,829 (+2) QPQQPFPQQPQ γ-gliadin/ω-gliadin 1 QQPFPQQPQ (438) DQ2.5 DQ2.5-glia-γ5
  597,799 (+2) PQQPQQPFPQ γ-gliadin/ω-gliadin 1 QQPQQPFPQ (472) DQ2.5/DQ8 DQ2.5-glia-γ4c/DQ8-glia-γ1a
  1198,585 (+1) TQQPQQPFPQ γ-gliadin 1 QQPQQPFPQ (472) DQ2.5/DQ8 DQ2.5-glia-γ4c/DQ8-glia-γ1a
  549,272 (+2) QQPQQPFPQ γ-gliadin/ω-gliadin 1 QQPQQPFPQ (472) DQ2.5/DQ8 DQ2.5-glia-γ4c/DQ8-glia-γ1a
  911,126 (+3) PQQPFPQPQLPYSQPQPFRPQQP α-gliadin 4 QPFPQPQLPY (42) DQ2 DQ2.5-glia-α1a
      PFPQPQLPY (53) DQ2.5 DQ2.5-glia-α1a
      QPFPQPQLPYSQ (164) DQ2 DQ2.5-glia-α1a
      PFPQPQLPYSQ (166) DQ2 DQ2.5-glia-α1a
  1253,631 (+2) PQQPFPQPQLPYSQPQPFRPQ α-gliadin 4 QPFPQPQLPY (42) DQ2 DQ2.5-glia-α1a
      PFPQPQLPY (53) DQ2.5 DQ2.5-glia-α1a
      QPFPQPQLPYSQ (164) DQ2 DQ2.5-glia-α1a
      PFPQPQLPYSQ (166) DQ2 DQ2.5-glia-α1a
  641,984 (+3) QPQPFRPQQPYPQSQP α/β-gliadin 1 FRPQQPYPQ (93) DQ2.5 DQ2.5-glia-α3
  541,937 (+3) QQPPFSQQQQPVLP LMW glutenin/γ-gliadin 1 PFSQQQQPV (706) DQ2.2 DQ2.2-glut-L1
  774,383 (+2) PQQPFPQQPQQPQ ω-gliadin 3 PQQPFPQQPQQP (195) DQ2 DQ2.5-glia-γ5
      PQQPFPQQPQQ (432) DQ2 DQ2.5-glia-γ5
      QQPFPQQPQ (438) DQ2.5 DQ2.5-glia-γ5
1 597,799 (+2) PQQPQQPFPQ γ-gliadin/ω-gliadin 1 QQPQQPFPQ (472) DQ2.5/DQ8 DQ2.5-glia-γ4c/DQ8-glia-γ1a
  1198,584 (+1) TQQPQQPFPQ γ-gliadin 1 QQPQQPFPQ (472) DQ2.5/DQ8 DQ2.5-glia-γ4c/DQ8-glia-γ1a
  549,273 (+2) QQPQQPFPQ γ-gliadin/ω-gliadin 1 QQPQQPFPQ (472) DQ2.5/DQ8 DQ2.5-glia-γ4c/DQ8-glia-γ1a
  597,945 (+3) PQQPQQPYPQQPQQP γ-gliadin 3 QQPQQPYPQ (458) DQ2.5/DQ8 DQ2.5-glia-γ3/DQ8-glia-γ1b
      QQPYPQQPQ (464) DQ2 (glia-γVIb)
      PYPQQPQQP (468) DQ2 (DQ2.2 and DQ2.5)
2 597,799 (+2) PQQPQQPFPQ γ-gliadin/ω-gliadin 1 QQPQQPFPQ (472) DQ2.5/DQ8 DQ2.5-glia-γ4c/DQ8-glia-γ1a
  1198,583 (+1) TQQPQQPFPQ γ-gliadin 1 QQPQQPFPQ (472) DQ2.5/DQ8 DQ2.5-glia-γ4c/DQ8-glia-γ1a
  549,273 (+2) QQPQQPFPQ γ-gliadin/ω-gliadin 1 QQPQQPFPQ (472) DQ2.5/DQ8 DQ2.5-glia-γ4c/DQ8-glia-γ1a
3 1198,585 (+1) TQQPQQPFPQ γ-gliadin 1 QQPQQPFPQ (472) DQ2.5/DQ8 DQ2.5-glia-γ4c/DQ8-glia-γ1a
  597,799 (+2) PQQPQQPFPQ γ-gliadin/ω-gliadin 1 QQPQQPFPQ (472) DQ2.5/DQ8 DQ2.5-glia-γ4c/DQ8-glia-γ1a
  541,936 (+3) QQPPFSQQQQPVLP LMW glutenin/γ-gliadin 1 PFSQQQQPV (706) DQ2.2 DQ2.2-glut-L1
  597,800 (+2) PQPQQQFPQP γ-gliadin 1 PQPQQQFPQ (577) DQ2.5 DQ2.5-glia-γ4b
4 597,799 (+2) PQQPQQPFPQ γ-gliadin/ω-gliadin 1 QQPQQPFPQ (472) DQ2.5/DQ8 DQ2.5-glia-γ4c/DQ8-glia-γ1a
  1198,586 (+1) TQQPQQPFPQ γ-gliadin 1 QQPQQPFPQ (472) DQ2.5/DQ8 DQ2.5-glia-γ4c/DQ8-glia-γ1a
  549,273 (+2) QQPQQPFPQ γ-gliadin/ω-gliadin 1 QQPQQPFPQ (472) DQ2.5/DQ8 DQ2.5-glia-γ4c/DQ8-glia-γ1a
  641,983 (+3) QPQPFRPQQPYPQSQP α/β-gliadin 1 FRPQQPYPQ (93) DQ2.5 DQ2.5-glia-α3
  541,936 (+3) QQPPFSQQQQPVLP LMW glutenin/γ-gliadin 1 PFSQQQQPV (706) DQ2.2 DQ2.2-glut-L1
5 710,354 (+2) PQQPQQPFPQPQ γ-gliadin/ω-gliadin 1 QQPQQPFPQ (472) DQ2.5/DQ8 DQ2.5-glia-γ4c/DQ8-glia-γ1a
  597,799 (+2) PQQPQQPFPQ γ-gliadin/ω-gliadin 1 QQPQQPFPQ (472) DQ2.5/DQ8 DQ2.5-glia-γ4c/DQ8-glia-γ1a
  541,936 (+3) QQPPFSQQQQPVLP LMW glutenin/γ-gliadin 1 PFSQQQQPV (706) DQ2.2 DQ2.2-glut-L1