EVs and monocytes of seropositive patients exhibit some similar characteristics between SF and blood. (A) From left to right; a picture depicting a monocyte agglomerate in SF from an anti-CCPhiRFhi patient. Representative CD14 and FMO for CD16 pseudo-color plots of monocyte subsets gated on CD45+HLA-DR+ cells from the SF of an anti-CCPhiRFhi patient. Monocyte subset frequencies in the SF from three seropositive patients. (B) Representative histograms of HLA-DR, CD86, CD36, CCR2, CX3CR1, and CCR5 of intermediate monocytes from the blood of a HC (gray), an anti-CCPhiRFhi patient (red), and the SF from the same patient (blue). (C) Collective t-SNE of EVs (1 × 105) derived from three samples of SF from seropositive RA patients analyzed and plotted. Every dot represents a single EV and the color indicates ArcSinh5-transformed expression values for the given parameter analyzed and calculated over EVs from all samples varying from blue for lower expression to red for higher expression.