Seropositive patients had high counts of intermediate monocytes. (A) Representative CD14 and CD16 heat map plots of monocyte subsets (CD14++CD16− (classical), CD14++CD16+(intermediate), and CD14+CD16++ (non-classical) monocytes) gated on CD45+HLA-DR+ cells from the total blood of 1 individual of each study group. (B) Frequencies (upper panels) and absolute counts (lower panels) of classical, intermediate, and non-classical monocytes from anti-CCP−RF−, anti-CCP+RF+/−, and anti-CCPhiRFhi RA patients, and HCs. (C) MFI of HLA-DR, CD86, CD36, CCR2, CX3CR1, and CCR5 on intermediate monocytes from anti-CCP−RF−, anti-CCP+RF+/−, anti-CCPhiRFhi RA patients, and HCs. Comparisons among the groups were made by performing the Kruskal–Wallis test and Dunn’s post-hoc test.