Tissue expression pattern of Ly75 and the causal inference test (CIT) of adjusted inguinal fat pad weight in KO/B6J and B6J/B6J mice of an F2 population between KO and B6J strains. (a) Ly75 expression in 10 tissues. Data are means ± S.E.M. for expression levels relative to the KO/B6J liver. Detailed data are shown in Supplementary Table S8. (b) Criteria for CIT16. Four component tests of CIT assess whether the change in genotype (G) leads to variation in a quantitative trait (T) via the change in Ly75 expression (E). (c) Result of Test 1. (d) Result of Test 2. (e) Result of Test 3. (f) Result of Test 4. In (c–f), data are least squares means ± S.E.M. P values were obtained from linear regression models of the component tests shown in (b).