Cell composition in DRG and developmental growth of the axons of primary sensory neurons in adult TRPV2flox/flox; Cre mice. (a) Representative immunofluorescence images of DRG from TRPV2flox/flox and TRPV2flox/flox; Cre mice stained for TRPV1 (marker for C-nociceptors), peripherin (for unmyelinated neurons), and NF200 (for myelinated neurons). Scale bar, 100 μm. (b,c) TRPV2 expression in DRGs from TRPV2-deficient mice (TRPV2flox/flox; Cre) and normal littermates (TRPV2flox/flox): (b) immunostaining, scale bar, 100 μm; (c) western blot. An upper panel in c, an anti-TRPV2 antibody-positive band; a lower plane, anti-Na+/K+-ATPase antibody-positive bands as loading controls. The same blot was reprobed for each staining and the appropriate areas were cropped. The original full-length images were shown in Supplementary Fig. S6. (d) Prevalence of each class of DRG neurons shown in (a) (>2000 cells from 6 mice). Open bars, TRPV2flox/flox; closed bar, TRPV2flox/flox; Cre. Data are mean ± S.E.M. (n = 6). (e) Cross-sectional electromicrographs of the sural nerve, which is dominated by sensory afferents innervating the hind paw. Scale bar: left panels, 50 μm; right panels, 5 μm. (f) The numbers of unmyelinated (UM), myelinated (M), and total fibers in a whole cross-section of the sural nerve (n = 3).