The role of tRFs in arsenite-induced inflammation. (A) A549 cells in 6-wells were transfected with 100 nM anti-sense oligos (anti-“tRF5-GluCTC”, -“tRF5-ProTGG”, -“tRF5-AlaCGC” or -“CN”). After 2 h post-transfection, cells were treated with 10 µM arsenite for 15 h. Untreated cells were used as controls. The nuclear fractions were prepared, followed by the SDS-PAGE gel running and Western blot, to detect the nuclear translocation of p65. (B) The supernatant of each group was harvested, and its IL-8 was measured by an ELISA kit from R&D (R&D system, Minneapolis, MN). Data shown are representative of three independent experiments. *P < 0.05 relative to CN oligo-treated cells.