Figure 5 | Scientific Reports

Figure 5

From: Protective effect of pre- and post-vitamin C treatments on UVB-irradiation-induced skin damage

Figure 5

Effects of AA on UVB-induced oxidative damage and ROS production. AA was applied at concentrations of 100 and 500 mM to the reconstituted human epidermal surface for 3 h before or after 120 mJ/cm2 UVB irradiation. After UVB irradiation, the epidermis was cultured for 24 h. (a) Immunofluorescence staining for 4-HNE as a maker of lipid peroxidation. White arrowheads indicate 4-HNE-positive cells. (b) ROS levels in the epidermis were detected by DHE staining. White arrowheads indicate ROS-positive cells. The dotted line represents the bottom of the cornified layer. Bar = 50 µm.

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