Acute inhibition of A6 neurons and effect on the inspiratory, active expiratory and ventilatory responses to stimulation of central chemoreceptors in conscious adult rats. (A) Raw and integrated (∫) records of DiaEMG and AbdEMG activities from one animal in which the LC was transduced with PRSx8-AlstR-GFP-LVV. Note the changes in inspiration, expiration and in the number of sighs (red lines) induced by activation of the central chemoreceptor (7% CO2), before and after Alst application into lateral ventricle. (B) Magnification of baseline and reflex inspiratory, expiratory and ventilatory (barometric respiratory movements) responses from the same rat before and after Alst application. Note that Alst application reduced significantly the fR, DiaEMG amplitude, the AbdEMG active expiration incidence (green arrow: absence of active expiration; red arrow: active expiration) and magnitude, as well as the ventilatory parameters.