1-Oxo-2,2,6,6-tetramethylpiperidinium bromide converts α-H N,N-dialkylhydroxylamines to nitrones via a two-electron oxidation mechanism

Herein we provide experimental proof that 1-oxo-2,2,6,6-tetramethylpiperidinium bromide converts α-H N,N-dialkylhydroxylamines to nitrones via a two-electron oxidation mechanism. The reactions reported are rapid, proceed under mild conditions, and afford nitrones in excellent yields.

profile by conventional EPR spectrometry. Similarly, the oxidation of the α-H hydroxylamines 4a-c (100 μM) by 1.Br (25 μM) proceeded with concomitant formation of nitroxide 2 (Fig. 3B, blue, red and black spectrum, respectively). As suggested by the constant magnitude of these spectra, each reaction was completed in less than 30 seconds, which is the approximate time required for sample preparation and data acquisition.
HPLC analysis of the reaction solutions revealed that the oxidation of 4a-c by 1 also led to formation of nitrones 6a-c ( Fig. 3C; Supplementary Information; SI). The requirement for a slight stoichiometric excess of   Open rectangles, 4b in the absence of 1 (incubation time, 20 min). The data are presented as mean values of three independent experiments ± the standard error. D-EPR spectra of 1.Br (125 μM) plus 4c (100 μM) before (blue trace) and after (black trace) addition of C 2 H 5 ONa (30 mM; red trace-100 μM standard solution of nitroxide 2). the oxoammonium salt for complete oxidation of 4a-c most likely reflected the competition between 4a-c as reactants and the end-reaction product 3 for 1. In support of this assumption, the oxidation of hydroxylamine 4b (100 μM) by 1 (125 μM) to nitrone 6b (98 μM; Fig. 3C) was paralleled by formation of both nitroxide 2 (14 μM; Similar distribution of the products was observed when reactions were carried out with 4a-c in CH 3 OH, C 2 H 5 OH, CH 3 CN, and CH 2 Cl 2 with the notion that the oxoammonium cation 1 does not react with CH 3 CN and CH 2 Cl 2 to any significant extent but does oxidize primary alcohols to aldehydes. Hence, in ethanol, the complete oxidation of hydroxylamine 4c to nitrone 6c required larger excess of 1 (Fig. 3C, filled rectangles). Ethanol, however, did not prevent the formation of 6c, which suggests that the formation of nitrones would be the preponderant process in polyfunctional compounds containing both OH and NOH groups. The stoichiometry of the reactions further suggests that nitrones were not formed via the intermediate formation and disproportionation of α-H nitroxides as completion of the latter process would require 2 molar equivalents of 1 for the oxidation of the hydroxylamines (Fig. 2). This conclusion was further supported by kinetic analyses of the decay of nitroxides 5b,c.
In Fig. 4A1 is shown the EPR spectrum of water containing ethanol (25%), ethylenediaminetetraacetic acid (EDTA; 100 μM), and hydroxylamine 4c (200 μM). Addition of NaOH (0.5 M) led to the appearance of the EPR spectrum of nitroxide 5c (in mT, a H = 1.0318; a N = 1.7607), which reflected the oxidation of the aminoxyl anion of 4c by oxygen 25 (Fig. 4A2; spectrum 3, computer simulation of the EPR spectrum of 5c). We then acidified the reaction solution to pH 6.0 with acetic acid and recorded both the decreases in the EPR spectrum of 5c (Figs 2B and 4A2, open circles; the spin concentration of 5c was determined by double integration of the EPR signals using authentic 2 as a standard) and the formation of nitrone 6c (as assessed by HPLC; Fig. 4B, filled circles). In agreement with Fig. 2, two molecules of 5c were consumed for each molecule of 6c formed in the reaction. Importantly, the half-life of 5c (t 1/2 ~ 60 minutes) largely exceeded the time required for oxidation of 4c by 1, thus excluding the generation and disproportionation of nitroxide 5c as a reaction mechanism responsible for the formation of nitrone 6c.
We further carried out experiments to verify whether nitroxide 5b is formed during the oxidation of hydroxylamine 4b by 1, with the expectation that the large difference in the hyperfine splitting constants of 2 and 5b 25 will allow their simultaneous EPR detection in the reaction milieu. In Fig. 5.1 is shown the EPR spectrum of 5b generated in an alkaline solution of 4b (in mT, a H = 1.097; a N = 1.761; spectrum 3, computer simulation of the EPR spectrum of 5b). In agreement with the data reported in ref. 25 , alkalization of the solution of 4b led to the appearance of the EPR spectrum of 5b, which increased for ~1 min and then remained constant for ~30 min. Acidification of the reaction solution to pH 6.0 and following kinetic analysis of the decay 5b established that the half-life of this radical is ~4 min (data not shown), which provides ample time for its EPR analysis. In Fig. 5.3 is presented the EPR spectrum of a reaction solution consisting of hydroxylamine 4b (1 mM) and 1. Br (1 mM), which contains as a major component the three spectral lines of 2 (red tracing). By comparing the latter spectrum with that of 5b as a standard (4.9 μM; blue tracing), we observed that 5b was present in the reaction solution at a submicromolar concentration, or less than 0.1% of the expected (~0.8 mM) for one-electron oxidation of 4b; in Fig. 5.3, the first two spectral lines of 5b are denoted with arrows.  The data presented in Fig. 6A indicate that the trace amounts of nitroxide 5b were formed via a secondary reaction in which the end reaction product 2 oxidized the parent hydroxylamine 4b (Fig. 6A; 4b + 2 → 5b + 3); the EPR spectrum of 5b (~1 μM) could be observed upon addition of nitroxide 2 (1 mM) to a solution of hydroxylamine 4b (1 mM; Fig. 6A, red tracing). Incubation of this reaction solution led to a slow formation of nitrone 6b ( Fig. 6B; yield of nitrone for 1 hour, 3%), presumably via disproportionation of 5b. While under these experimental conditions reaction 4b + 1 → 6b was completed in less than 1 minute (Fig. 1C), the data presented in Fig. 6B indicate that, when the oxoammonium salt 1 was used as an oxidant, the secondary oxidation of the hydroxylamine did not significantly contribute to the formation of nitrone 6b.
Altogether, the data obtained support a two-electron oxidation mechanism for the reaction between the α-H N,N-dialkylhydroxylamines and 1, which is reminiscent of the oxidation of alcohols by oxoammonium salts 20 (Fig. 7). Accordingly, the stoichiometric oxidation of 4a-c by 1 is proposed to proceed via formation of reaction intermediates 7a-c with concomitant cyclic elimination of the end-reaction products 6a-c and 3. α-H N,N-dialkylhydroxylamines by 1.Br under preparative conditions. Nitrones are widely used as reagents in reactions of cycloaddition and alkylation with organometalics, and as EPR spin-trapping probes. Hence, considerable research effort has been directed toward the synthesis of this class of compounds. The oxidation of α-H N,N-dialkylhydroxylamines has proven a principal method for the synthesis of nitrones, where HgO 30 , Ag 2 O 31 , MnO 2 32 , hypervalent iodine reagents 33 , and copper complexes 34 have been successfully used as oxidants. An alternative that uses nontoxic reagents has been reported by Alderson et al. 35 and Cicchi et al. 36 in which NaBrO and NaClO oxidize α-H N,N-dialkylhydroxylamines to nitrones with reaction times and yields ranging from 1 to 20 hours and 40% to 95%, respectively.

Oxidation of
The high rates of oxidation of α-H N,N-dialkylhydroxyl-amines by 1 and the excellent yields of nitrones under analytical conditions prompted us to scale-up the reactions to preparative amounts of hydroxylamines. At ambient temperature, 1-2 mmoles of 4a-e (dissolved in 90% methanol) were efficiently oxidized by 1.Br (Fig. 8). Hydroxylamines 4a,b,d are standard substrates of oxidation and provide a foundation for comparison of different synthetic protocols; depending on the oxidant used, reactions with 4a,b,d have been reported to proceed for hours and to afford nitrones  with good to excellent yields 33,34,36,37 . In agreement with the data presented in Fig. 3C, maximal yields of nitrones were obtained with the use of 1.2 molar equivalents of 1.Br per mole of hydroxylamine. In the absence of NaHCO 3 the reactions proceeded with concomitant hydrolysis of the nitrones by HBr to aldehydes and N-alkylamines (data not shown). In the oxidation of 4d, formation of 6dd was not detected, whereas 4e afforded regioisomers 6e (yield, 78%) and 6ee (yield, 14%; SI). Notably, 1 selectively oxidized the NOH of 4e and not its OH group. Under these experimental conditions, oximes 8a,b did not react with 1 to any significant extent, indicating that the reaction is specific for hydroxylamines and that deprotonation of the NOH group did not promote its oxidation, presumably via preferential addition of the corresponding aminoxyl anions to 1. As estimated with MarvinSketch (ChemAxon; Cambridge, MA), in aqueous solutions the pKa values of the NOH group of 4b, 8a and 8b are 15.57, 9.84 and 7.37, respectively.

Conclusions
The data presented herein expand the list of functional groups that can be interconverted by oxoammonium salts. We show that 1 converts the >N-OH group into a nitrone group via a two-electron oxidation mechanism in each of a series of α-H dialkylhydroxylamines. The reaction is rapid, proceeds under mild conditions, and affords nitrones in excellent yields.
The interconversion between nitrones and hydroxylamines is a viable strategy for carbon-carbon formation that, when coupled with acidic hydrolysis of nitrone derivatives, can be applied to structural diversification of aldehydes [38][39][40][41][42][43] . To this end, the rapid oxidation of α-H N,N-dialkylhydroxylamines by oxoammonium salts may prove advantageous to the optimization of one-pot synthetic protocols. Since dealkylation of α-H N,N-dialkylhydroxylamines may also be of interest, the oxidation of this class of compounds by 1.Br to nitrones can be followed by acid-catalyzed hydrolysis of the latter, which would afford aldehydes (or ketones) and N-alkylhydroxylamines.

Materials and Methods
Reagents. Hydroxylamines 4a,b were purchased from TCI America, Inc. (Montgomeryville, PA). All other chemicals, including nitrones 6b,c,d were purchased from Sigma (St. Louis, MO). Nitrone 6a was obtained via oxidation of 4a with Ag 2 O as reported in ref. 31 . Nitrones 6a-d were used as external reference HPLC standards. Protocols for preparation of 1.Br, 3 and 8a,e are included in SI, along with NMR, HRMS, and HPLC data.
General procedure for the oxidation of α-H N,N-dialkylhydroxylamines by 1.Br. To a stirred suspension of α-H N,N-dialkylhydroxylamine (1 mmol) in methanol containing 10% water (v/v; 10 mL; 25 °C) and NaHCO 3 (250 mg; 3 mmol) was added dropwise 1.25 equiv. of 1.Br (dissolved in 10 mL CH 3 CN) over 5 min. Instant decolourisation of the dark-brown solution of 1.Br followed the addition without any apparent effervescence. The inorganic salts were filtered off, washed with absolute ethanol (2 × 5 mL), and the solvents from the filtrate were rotor-evaporated (35 °C; 20 Torr). Nitrones from the dry residues were separated by column chromatography as indicated SI.