Figure 5 | Scientific Reports

Figure 5

From: Simultaneous evaluation of anti-EGFR-induced tumour and adverse skin effects in a microfluidic human 3D co-culture model

Figure 5

Effects of cetuximab treatment on skin microtissue at day 5 of co-culture. (a) H&E staining of untreated control group indicating maintained columnar basal keratinocyte layer. (b) Immunofluorescent TUNEL (green) and Ki67 (red, arrows) staining of untreated control group indicating proliferative keratinocytes in the basal layer. (c) H&E staining of antibody-exposed group showing irregular arrangement of keratinocytes in the stratum basale. (d) Immunofluorescent TUNEL (green) and Ki67 (red) staining of antibody-exposed group demonstrating complete lack of proliferative cells. (a,c) Dotted lines (orange) framing keratinocytes inside the stratum basale. (b,d) Nuclei stained with DAPI (blue). Scale bar: 100 µm. (e) Gene expression of tumour necrosis factor alpha (TNFα) and Keratin 1 (KRT1) in skin microtissue of untreated control group (white bars) and antibody exposure group (black bars), n = 3. Data shown as mean + SD.