Figure 3 | Scientific Reports

Figure 3

From: Simultaneous evaluation of anti-EGFR-induced tumour and adverse skin effects in a microfluidic human 3D co-culture model

Figure 3

Static monolayer tumour proliferation assay (a) and tumour behaviour in five-day chip co-culture (b–d). (a) Dose-dependent cytotoxicity of cetuximab on H292 cells in static monolayer culture, n = 6. (b) Co-culture regimen illustrating total media volume exchange (), half media volume exchange () and antibody exposure time points (). (c) Representative tumour culture compartment section illustrating a typical arrangement of tumour spheroids at day 0 and 3D fried egg shape after five days. Scale bar: 500 µm. (d) LDH release in untreated control group (white bars) and in antibody exposure group (black bars) over five days of chip culture, n = 4. *p < 0.05 using unpaired two-tailed Student’s t test. Data shown as mean + SD.