Figure 2 | Scientific Reports

Figure 2

From: Simultaneous evaluation of anti-EGFR-induced tumour and adverse skin effects in a microfluidic human 3D co-culture model

Figure 2

Morphology of in vitro generated skin microtissue (ac) and tumour microtissue (df). (a) Haematoxylin and eosin (H&E) staining of the physiological architecture of a human full-thickness skin microtissue on day 16 of air–liquid culture under static conditions showing the columnar basal keratinocyte layer, a stratified stratum corneum and the layers in-between. (b) Immunofluorescent TUNEL (green) and Ki67 (red) staining indicating proliferative keratinocyte stem cells in the basal layer (red) and the apoptotic (cornifying) cells (green) on the upper skin layer. (c) Collagen IV staining of the basement membrane of the skin. (d) H&E staining of homogeneously distributed identical H292 cells throughout the tumour spheroid. (e) Immunofluorescent TUNEL (green) and Ki67 (red) staining indicating healthy tumour microtissue status containing a significant fraction of growing tumour cells (red). (f) Vimentin (red) staining of the tumour microtissue showing fibroblastic properties. (b,c,e,f) Nuclei stained with DAPI (blue). Scale bar: 100 µm.