Table 1 Combining HGD65 concentration estimates from separate experiments.

From: A handheld platform for target protein detection and quantification using disposable nanopore strips

Nanopores* Target Conc. (nM) Combined Estim. (nM) CVь PEь
P7, P8 0.1 0.138 ± 0.002 1.09% 38.2%
P7, P8 0.2 0.197 ± 0.027 13.6% −1.71%
P9, P10, P12, P13 0.5 0.612 ± 0.17 27.6% 22.4%
P9-P13 1 0.927 ± 0.07 7.69% −7.33%
P10-P13 2.5 2.9 ± 0.59 20.3% 16.1%
P10, P12, P13 5 5.3 ± 2.32 43.7% 5.91%
P14, P15 1.4 1.34 ± 0.143 10.7% −4.58%
P14, P15 2.8 2.88 ± 0.145 5.02% 2.79%
P21-P23, P25, P26 0.266 0.278 ± 0.135 48.6% 4.6%
P22-P26 0.665 0.684 ± 0.176 25.8% 2.8%
P21, P23, P25, P26 1.33 1.16 ± 0.245 21% −12.5%
Summary    20.5% |10.8|%
  1. *Pores were grouped together by a common detection reagent (pores P7-P13 with 1074 bp, pores P14-P15 with 217 bp), or by the presence of saliva background (pores P21-P26, 10% saliva at incubation, 1% saliva above the pore after 10X dilution).
  2. Target concentrations are above the nanopore after dilution. The pre-dilution incubation concentrations are reported in Table 2.
  3. Combined estimates are the mean and standard deviation of the single-pore estimates at each target concentration. The quantitative estimates for the individual pores listed are reported in the Supplementary Tables and plotted in Supplementary Fig. S22.
  4. ьCoefficient of variation (CV) is the standard deviation divided by the mean. Percent error (PE) is the ratio of the error (estimate minus known value) to the known value, converted to a percentage. Summary reports the mean CV and the mean of the absolute PE values across all estimates.