Comparison of the most abundantly expressed ASFV proteins. Protein proportions were calculated as mole percentages (x-axes) on basis of the tryptic digests using the emPAI algorithm. Panel A shows the 20 most abundant proteins (including cellular proteins as grey bars) and panel B the 20 most abundant ASFV proteins in decreasing order. Even in HEK 293 cells, which had the lowest overall content of ASFV proteins, two viral proteins were present among the 20 most abundant proteins. In all cell lines the structural protein p11.5 as well as the so far uncharacterized proteins pK145R and pI73R were expressed at high abundance. Note that in panel B, despite of some overlap between the top-ranking proteins in different cells, there are considerable differences in the abundances.