Figure 4 | Scientific Reports

Figure 4

From: Involvement of DHH and GLI1 in adrenocortical autograft regeneration in rats

Figure 4

IHC and in situ hybridisation in the adrenocortical autograft at POD7. (A) Cyp11b1 expression with in situ hybridisation using Basescope. Cyp11b1 was detected in remnant adrenocortical cells. (B) CYP11B2 expression with IHC. CYP11B2 signals are indicated as red and autofluorescence as white. No CYP11B2 was found. (C) DAB2 expression with IHC. (D) PCNA expression with IHC. (E) Gli1 with in situ hybridisation using RNAscope. (F) Shh with in situ hybridisation using RNAscope. (G) Dhh with in situ hybridisation using RNAscope. (H) Ihh with in situ hybridisation using RNAscope. In panels E–H, one dot signal represents one mRNA copy in the RNAscope results. RNAscope signals are represented as white colour dots and autofluorescences were subtracted from original figures (Supplementary Fig. 3). AC: adrenocortical cells.

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