Table 1 Influenza rA/Puerto Rico/8/1934 virus, a common laboratory strain and candidate vaccine virus backbone, was used in this study to demonstrate direct RNA sequencing effectiveness and repeatability using a crude starting material.

From: Direct RNA Sequencing of the Coding Complete Influenza A Virus Genome

Influenza A Virus Subtype Volume Titer* Purpose in Study
rA/Puerto Rico/8/1934 H1N1 200 µL 4.2 × 1011 Pure Virus
rA/Puerto Rico/8/1934 H1N1 1,500 µL 6.8 × 109 Crude Virus Triplicates
A/Florida/20/2018 H1N1pdm09 2,100 µL 3.2 × 107 Contemporary virus & LOD
A/Texas/50/2012 H3N2 500 µL 3.5 × 106 Contemporary virus
A/chicken Ghana/20/2015 HPAI H5N1 500 µL 4.3 × 107 Contemporary virus
A/British Columbia/1/2015 LPAI H7N9 500 µL 3.2 × 108 Contemporary virus
  1. Influenza A/Florida/20/2018 (H1N1pdm09), A/Texas/50/2012 (H3N2), A/chicken Ghana/20/2015 (HPAI H5N1) and A/British Columbia/1/2015 (LPAI H7N9) viruses were used to demonstrate this method’s broad utility across contemporary influenza A viruses of current clinical significance. Influenza A/Florida/20/2018 (H1N1pdm09) virus was also used to determine the limit of detection.
  2. *Influenza A/Florida/20/2018 (H1N1pdm09), A/Texas/50/2012 (H3N2), and A/British Columbia/1/2015 (LPAI H7N9) viruses were propagated in MDCK cells and the titer is presented as a TCID50. Influenza rA/Puerto Rico/8/1934 (H1N1) and A/chicken Ghana/20/2015 (HPAI H5N1) viruses were propagated in embryonated chicken eggs and the titers are presented as EID5050.