Figure 4 | Scientific Reports

Figure 4

From: Direct RNA Sequencing of the Coding Complete Influenza A Virus Genome

Figure 4

The aligned read length distributions correspond to the expected lengths (dashed lines) of the respective segments (NS 890 nt; M 1,027 nt; NA 1,413 nt; NP 1,565 nt; HA 1,778 nt; PA 2,233 nt; PB1 and PB2 2,341 nt) from the influenza rA/Puerto Rico/8/1934 (H1N1) crude viral samples. As the segment length increases, the read length distribution falls further short of the expected length, presumably due to RNA degradation. Aligned read lengths include insertion errors, accounting for the presence of reads larger than the expected value. Due to cases of large insertion errors, 14 total reads longer than 2,500 nucleotides were observed.

Back to article page