(A) Influenza A viruses contain highly conserved 12 and 13 nt sequences at the 3′ and 5′ termini. (B) The key component of Oxford Nanopore direct RNA sequencing is a Reverse Transcriptase Adapter (RTA) which targets poly(A) mRNA and is ligated to the 3′ end of the mRNA. A sequencing adapter is then ligated to the RTA which directs the RNA strand into the pore for sequencing. (C) The RTA was modified to target the 3′ conserved 12 nt of the influenza A virus genome. (D) The modified RTA hybridizes and is ligated to vRNA in the first step of direct RNA sequencing.