Novel Clade C-I Clostridium difficile strains escape diagnostic tests, differ in pathogenicity potential and carry toxins on extrachromosomal elements

The population structure of Clostridium difficile currently comprises eight major genomic clades. For the highly divergent C-I clade, only two toxigenic strains have been reported, which lack the tcdA and tcdC genes and carry a complete locus for the binary toxin (CDT) next to an atypical TcdB monotoxin pathogenicity locus (PaLoc). As part of a routine surveillance of C. difficile in stool samples from diarrheic human patients, we discovered three isolates that consistently gave negative results in a PCR-based screening for tcdC. Through phenotypic assays, whole-genome sequencing, experiments in cell cultures, and infection biomodels we show that these three isolates (i) escape common laboratory diagnostic procedures, (ii) represent new ribotypes, PFGE-types, and sequence types within the Clade C-I, (iii) carry chromosomal or plasmidal TcdBs that induce classical or variant cytopathic effects (CPE), and (iv) cause different levels of cytotoxicity and hamster mortality rates. These results show that new strains of C. difficile can be detected by more refined techniques and raise questions on the origin, evolution, and distribution of the toxin loci of C. difficile and the mechanisms by which this emerging pathogen causes disease.

In silico confirmation of toxin loci. Mapping Illumina reads to the genome of the reference strain 630 confirmed that HMX-149, HMX-152, and HSJD-312 lack tcdA and tcdC ( Fig. 2A) and revealed that the PaLoc subtypes in HMX-152 and HSJD-312 are closely related. HMX-149, instead, has a different PaLoc structure and integration site ( Fig. 2A).
Mapping also demonstrated that HMX-152 and HSJD-312 carry full cdtR, cdtA, and cdtB alleles (Fig. 2B). By contrast, the reads of isolate HMX-149 only mapped to the 5′ end of cdtR and to the 3′ end of cdtB in the genome of reference strain R20291 (Fig. 2B), confirming that it lacks the binary toxin.
The ribotypes obtained for isolates HMX-149, HMX-152, and HSJD-312 are new in the Canada NML database (NML210, NML211, NML212). Moreover, isolates HMX-149 and HSJD-312 were linked to unseen PFGE macrorestriction patterns in the same reference center (NML-1108 and NML-1109, respectively). By in silico restriction analysis of the B1 fragment of tcdB, we classified HMX-152 and HSJD-312 as toxinotype XXXI and gathered evidence to classify isolate HMX-149 to a new toxinotype (Fig. 3C). A PCR-approach, instead, confirmed their identification as C. difficile (tpi + ) and revealed that they carry tcdB and in two cases cdtB (lower panel). The three isolates are negative for tcdC. A control NAP1 strain was tested for comparative purposes. Two multiplex PCR reactions were performed, hence two agarose gels appear in the figure. The first reaction targets cdtB, tcdA, and tdc. The second amplifies tcdC and tpi.  All hamsters inoculated with spores from the control NAP1 strain or HSJD-312 died in 4 or 7 days, respectively (Fig. 4B). By contrast, HMX-149 and HMX-152 only induced 20% of mortality (Fig. 4B).
Though HMX-149 showed more TcdB than a hyperproducing NAP1 strain in an SDS-gel (Fig. 5A), none of the TcdBs from our Clade C-I isolates were detected on a Western Blot by a monoclonal antibody that recognizes TcdB R20291 and TcdB 630 (Fig. 5A). RhoA was not detected by an antibody that does not recognize the glycosylated form of this small GTPase in cells exposed to supernatants of HMX-149, HMX-152, and HSJD-312 (Fig. 5B), suggesting that RhoA is a substrate of TcdBs from HMX-149, HMX-152, and HSJD-312 strains.
A Bayesian approach to test the hypothesis of a common origin for the antibody non-reactive TcdBs of the Clade C-I isolates and TcdB R20291 rendered a negative result (Fig. 6). Instead, it supported the notion that the antibody-escaping feature of TcdB HMX-149 and TcdB HMX-152/HSJD-312 is a product of convergent evolution. This approach also revealed that most of the amino acid residues under positive selection in these TcdBs are located in their GT domains (Fig. 6).
A comparison of structural models of TcdB R20291 , TcdB HMX-149 , and TcdB HHMX-152/HSJD-312 in search of epitopes that could explain the antibody-escaping feature of the Clade C-I toxins in the rapid tests did not reveal major variations, except for two subtle differences in the CROPs region (D(2091)N and D(2094)N, Supplementary  Fig. 2). CdtLoc features. The CdtA and CdtB amino acid sequences of HMX-152, HSJD-312, and the CDT + Clade C-I strains SA10-050 and C10-165 were highly conserved (98-99% AAI). These sequences only showed 71% or 80% identity to the CdtA and CdtB sequences of strain R20291, respectively ( Supplementary Fig. 3). These differences were distributed across the entire CdtA sequence or predominantly restricted to the activation and receptor  binding domains of CdtB ( Supplementary Fig. 3). Relative to CdtA R20291 , all five Clade C-I CdtA sequences compared share a deletion of six amino acid residues after residue 8, two single residue insertions after residues 28 and 41, and an I309L substitution. A similar analysis using CdtB R20291 as reference revealed an insertion of four amino acid residues at position 814 in all Clade C-I isolates as well as a substitution of the C-terminal motif LSVD in strain R20291 by the sequence YEYHK. Moreover, the Clade C-I CdtB sequences analyzed have an asparagine residue at position 209 instead of the classical lysine residue.
PaLoc structures. Although HMX-149, HMX-152, and HSJD-312 have chromosomal cdu1, cdd2, and cdd3 genes, only HMX-149 has a monotoxin PaLoc inserted at this location (Fig. 7). Compared to C. difficile 630, the chromosomal PaLoc of HMX-149 lacks a small gene for a hypothetical protein, tcdA, tcdC, and cdd1, but shows an insertion of three putative CDS between tcdE and cdd2 (Fig. 7). This insertion was also seen in isolates HMX-152 and HJSD-312 (Fig. 7). Alike the non-toxigenic Clade C-I strain H5078, HMX-152 has five CDS between this sequence stretch and cdu1 (Fig. 7). We cannot provide information on the chromosomal insertion site of the PaLoc HMX-149 because it was found close to a contig end in our draft assembly. On the other hand, when we compared the PaLoc insertion site of our toxinotype XXXI isolates HMX-152 and HSJD-312 with that of the type strain WA151 (Clade 5), we found that the three isolates share a 10 bp sequence by immediately upstream of tcdR (GGATGATTTT). These three PaLoc sequences show >90% of identity across tcdR and tcdB, but start to differ in tcdE. The first gap in the alignment appears a few nucleotides before the 3′ end of tcdE in WA151, downstream from a AT dinucleotide.
The monotoxin PaLocs of HMX-152 and HSJD-312 include cwlH, tcdE, tcdB, and tcdR and are preceded by a transposase of the PD-(D/E)XK nuclease family and three genes for hypothetical proteins (Fig. 8). In both strains, we found a full CdtLoc composed of cdtR, cdtA, and cdtB downstream tcdR, followed by a gene for an integrase (Fig. 8). HMX-152 and HSJD-312 lack the lantibiotic synthesis and transport system that strain CD10-165 and the non-toxigenic Clade C-I strain RPH97 have upstream of this integrase (Fig. 8).
PaLoc carriage on putative plasmids. The toxin loci of HMX-152 and HSJD-312 are encoded on circular extrachromosomal molecules. These putative MGEs resemble conjugative plasmids because they have genes for i) a replication initiation protein, ii) proteins of a type IV secretory system, iii) various proteins with AAA-like domains, including a TraE homologue, iv) partition proteins, and v) helicases, a primase, a DNA polymerase III,   Table 1 and Fig. 9). IslandViewer4 did not detect genomic islands on the circular contigs of HMX-152 and HSJD-312. Nonetheless, AlienHunter identified their atypical PaLocs, but not their CdtLocs, as a horizontal gene transfer events.
The putative conjugative plasmids of HMX-152 and HSJD-312 also include a full agr locus, a tad-like locus, and putative virulence factors such as a chitinase and cell-wall binding proteins (Supplementary Table 1 and Fig. 9). These molecules are not related to toxin plasmids of C. perfringens or C. sordellii, including those of strains UMC2, JGS6364 and ATCC9714 (data not shown).
Through de novo assembly and read mapping to a closed PacBio sequence of the circular extrachromosomal element of isolate HSJD-312 we confirmed that the Clade C-I strains CD10-165, SA10-050, and RPH97 also carry related circular extrachromosomal molecules. However, they differ through gain or loss of various gene blocks, including the PaLoc, CdtLoc and Agr loci (Fig. 9).

Discussion
Here we describe three C. difficile strains isolated from human patients and by a detailed polyphasic characterization show that they are unique regarding their classification and toxin loci structures and locations. Moreover, we provide phenotypic data on the pathogenic potential of Clade C-I strains. Altogether, our findings reinforce the notion that the epidemiology of this emerging pathogen is not fully understood and deliver new information on the evolution of the PaLoc and CdtLoc in C. difficile. Moreover, since strains HMX-149, HMX-152, and  HSJD-312 escape common diagnostic techniques, our findings justify the development of more comprehensive tests for fast and adequate detection of Clade C-I strains in clinics and continuous typing and biological characterization of new isolates to ensure that such strains do not remain unnoticed. Similar to the Clade C-I strains SA10-050 and CD10-165 from France 15 , our isolates were shown by molecular techniques and NGS to be tcdA − , tcdC − , tcdB + and to have an endolysin with N-acetylmuramoyl-L-alanine amidase activity next to tcdE. We expand this previous knowledge by demonstrating that toxigenic Clade C-I strains may have different TcdB alleles, induce classical or variant CPE types, and cause distinct levels of cytotoxicity and hamster mortalities.
To our surprise, two strains with very similar PaLoc and CdtLoc structures markedly differed with respect to the biological activity of their supernatants (HMX-152 and HSJD-312), raising questions on their pathogenicity mechanisms. Additionally, we demonstrate that toxigenic Clade C-I strains may lack the binary toxin CDT and that CDT carriage did not lead to increased cytotoxicity or lethal activity.
Although we cannot reconstruct the PaLoc acquisition in strains HMX-149, HMX-152, and HSJD-312, their TcdB sequences are related to those of various strains from the Clade 2, which includes R20291 and other epidemic NAP1 strains. We provide indirect evidence that RhoA is glycosylated by them, irrespective of the type of CPE that they induce. This result is intriguing because vTcdBs seldom glycosylate Rho 16 . We cannot explain at this point how TcdB HMX-152 and TcdB HSJD-312 may target this small monomeric GTPase even though their GT domains notoriously differ from the GT domains of TcdB R20291 and other classical TcdBs that typically target RhoA. However, it would be interesting to decipher whether this unexpected ability is related to the positive selection that these GT domains seem to be experiencing. If so, it would be tempting to speculate that vTcdBs are more ancestral and that it has been more advantageous for C. difficile to exchange them for TcdBs exerting arborizing CPE and to complement them with TcdA, which has been shown to modulate the activity of TcdB 17 . In support of this hypothesis, some strains of C. sordellii, which as C. difficile are classified in the Cluster XI of the Clostridia and share biological and immunological traits with C. difficile 18 , have toxins in PaLoc-like elements that induce variant CPEs 19 .
To further add controversy to our current understanding of the pathogenesis of CDI, HMX-149 was linked to lower cell cytotoxicity and hamster mortality figures than a NAP1 strain though it noticeably produced more TcdB in vitro. This finding does not match the widespread notion that hypervirulence is related to TcdB overproduction 20 , but in vivo toxin measurements are required to confirm it.
The structural differences observed among the predicted TcdB 3D structures of strains HMX-149, HMX-152, and HSJD-312 and R20291 were traced back to the residues D(2091)N and D(2094)N, which are located within the contact site of a TcdB neutralizing antibody with therapeutic potential 21 . Based on their divergence from more prevalent C. difficile strains, it would be interesting to clarify whether these and other Clade C-I isolates are irresponsive to this type of intervention and therefore require alternative control strategies.
Compared to CDT R20291 , the binary toxin of toxigenic Clade C-I strains showed marked sequence differences in CdtA and the activation and receptor binding domains of CdtB, as well as deletions and substitutions of single residues and residue blocks. Therefore, it would be relevant to explore whether they are active or show distinct activities. In this regard, the K209D substitution observed in the CdtB sequences of the Clade C-I strains might impair their activation, as this is likely the cleavage site for serine-type proteases 11 .
Based on the reminiscence of the PaLocs of strains HMX-149 and ES130 (TcdA − TcdB + ) 22 and the sequence shared by HMX-152, HSJD-312 and the toxinotype XXXI strain WA151 upstream of tcdR, we postulate the Clade 5 as a possible PaLoc source for some Clade C-I isolates. So far, all toxin genes of C. difficile have been found in its chromosome 20 . Surprisingly, the PaLoc and CdtLoc of HMX-152 and HSJD-312 are encoded on a variety of extrachromosomal circular molecules, similar to the LCT toxins TcsL and TcsH of C. sordellii 23 . This group of putative plasmids is not restricted to Clade C-I strains, as we recently found another member in a C. difficile isolate ST-154 from the Clade 2 (unpublished data). These putative conjugative plasmids show no matches to sequences deposited in public databases, yet based on their annotation we anticipate that they encode additional virulence factors and an agr loci, possibly for toxin regulation 24 . This finding confirms the mobility of the CdtLoc, which was recently found in an episomal C. difficile bacteriophage 25 .
Besides the mobility that these putative plasmids could confer to their toxin loci, their monotoxin PaLocs were flanked by a transposase and an integrase. This scenario raises questions about the species distribution of TcdB and CDT, as their encoding genes could be present in yet-to-describe hosts. To further sustain this assumption, there are tcdA-and tcdB-related genes in C. novyi, C. perfringens, and C. sordellii and it has been claimed that the C. difficile PaLoc arose by interspecific recombination 13 . Linked to this issue, we urge a systematic examination of the taxonomic position of the Clade C-I of C. difficile, as the DNA-DNA hybridization values obtained for our strains were below the 70% threshold commonly used for species delimitation.
As to the antibiotic-susceptibility of strains HMX-149, HMX-152, and HSJD-312, we assayed ciprofloxacin and moxifloxacin because fluoroquinolones (FQ) have been linked to CDI development 26 and also because FQ-resistance might have facilitated the spread of epidemic C. difficile strains 27 . Likewise, rifampicin was tested because it has been postulated as a marker of epidemic strains 28 . The three Clade C-I strains studied are susceptible to these drugs. Nonetheless, we cannot assume that they are not epidemic based on their potential underrepresentation in isolate collections due to the difficulties linked to their detection in clinical laboratories.
Regardless of the severity of CDI induced by HMX-149, HMX-152, and HSJD-312, their isolation from clinical cases along with the unusual conformation and location of their PaLoc suggest that the minimum requirement for the induction of these infections is the ability to secrete at least one toxin targeting small GTPases of the Rho family. Under this scenario, it is expected that C. difficile continues its evolution and opens the possibility of the emergence of strains with the ability to cause severe disease in immunocompetent and non-compromised individuals. These concerns call for a continued surveillance on this intriguing pathogen using comprehensive genomic approaches. Methods C. difficile isolation and identification. Stool samples from patients admitted between 11/2011 and 11/2013 to the Costa Rican hospitals San Juan de Dios (HSJD) or Mexico (HMX) that developed antibiotic-associated diarrhea were screened for the presence of tcdA, tcdB, and ctdA/B using the Xpert C. difficile Epi assay (Cepheid). Positive samples were sent to the Laboratory for Anaerobic Bacteriology (LIBA) of the University of Costa Rica, where C. difficile was isolated and manipulated in a BSL2 facility. To this end, samples were treated with ethanol to eliminate vegetative cells and thereafter streaked onto CCFA plates (Oxoid). After five days of incubation under an atmosphere of 90% N 2 , 5% H 2 , and 5% CO 2 in an anaerobic chamber (Bactron, Shellab), yellow ground-glass colonies were subcultured under anaerobic conditions onto Brucella Agar plates supplemented with 5% laked blood, hemin, and vitamin K (BAK). After 2 days, colonies showing yellow-green fluorescence under UV light, positive results in a latex agglutination test (C. difficile Test Kit; Oxoid), and typical Gram-staining were identified using a PCR protocol that targets fragments of tpi, tcdA, tcdB, tcdC, and cdtB 29 .
Once identified as C. difficile, three tcdC − isolates were cryopreserved at −80 °C in BHI broth (Oxoid) supplemented with 15% glycerol and further examined. We are aware of the fact that Clostridium difficile was recently reclassified as Clostridiodes difficile 30 . However, we use the former name in this work because the majority of the scientific community continues to do so.

Rapid tests for toxin detection.
Besides the PCR-approach described above for C. difficile identification, we used the C. DIFF QUIK CHECK COMPLETE ® test (Techlab) for immunological detection of toxins A/B and the glutamate dehydrogenase antigen. Likewise, a helicase-dependent amplification test was used for qualitative detection of tcdA (AmpliVue C. difficile Assay/Quidel). Both systems were used according to the manufacturer's instructions.
Whole genome sequencing and bioinformatic analyses. Genomic DNA was obtained with the DNEasy Isolation Kit (Qiagen) from 48 h cultures of HMX-149, HMX-152, and HSJD-312 in Trypticase soy broth (Oxoid). The quality and yield of these preparations were verified by agarose gel electrophoresis and UV-spectrophotometry using a Nanodrop 2000 (Thermo Fischer Scientific). 2 × 250 bp paired-end reads were obtained through sequencing by synthesis at MicrobesNG (Birmingham, UK). After filtering (-q 30 -p 95) and artifact removal with the Fastx-toolkit (ver 0.0.14), preprocessed reads were assembled using SPAdes (v3. 10) or Edena (v3.131028). To resolve the structure of the extrachromosomal circular molecules of HSJD-312 and HMX-152, the genome of HSJD-312 was sequenced on a PacBio RSII instrument (Pacific Biosciences) using P6 chemistry. The obtained single-molecule real-time (SMRT) reads were assembled using the "RS_HGAP_ Assembly.3 Protocol" included in SMRT-Portal. The plasmidal contig (145122 bp) was circularized and adjusted to start with the repA genes. After long-read correction using the "RS_Bridgemapper.1" SMRT-Portal protocol, the plasmid sequence of HSJD-312 was short-read corrected by using Illumina paired-end reads. Prokka (ver 1.11) was used for automated annotation against databases containing C. difficile genomes from reference strains. To improve the annotation of the coding sequences (CDS) identified in the plasmid of HSJD-312 we used BLAST, BLASTP, PSIBLAST, BYPASS, PRODOM, SMART, and UniProt searches. Crunch files for comparison of specific loci were generated with WebACT using embl files as input. To determine the structure of the PaLoc and CdtLoc of HMX-149, HMX-152 and HSJD-312, Illumina reads were mapped with Bowtie2 (v.2.3.3.1) and BWA (v. 0.7.16a-r1181) to cognate regions in the reference strains 630 (CP010905.2) and R20291 (FN545816). All genomes and genome comparisons were visualized using Artemis or ACT. Linear and circular comparison figures were prepared with Easyfig and BRIG, in that order. For digital DNA-DNA hybridization, we used the GGDC 2.1 web server (http://ggdc.dsmz.de) and WGS fasta files for strains 630 (CP010905.2), R20291 (FN545816), M68 (N668375.1) and M120 (FN665653.1). Contig files were scanned against the C. difficile PubMLST typing scheme using MLST [https://github.com/tseemann/mlst]. The toxin contigs of HMX-152 and HSJD-312 were analyzed with IslandViewer4 for prediction of genomic islands and with AlienHunter for identification of lateral gene transfer events through the implementation of interpolated variable order motifs. Furthermore, we used MUSCLE 31 to generate alignments of extracted nucleotide or protein sequences and PhyML 32 and Figtree to transform the alignments into dendrograms.
Genotyping. We followed standard operating procedures and used databases from the National Microbiology Laboratory of the Public Health Agency of Canada (NML-PHAC) to assign SmaI PFGE macrorestriction patterns and ribotypes to the strains. For in silico toxinotyping, we extracted the B1 fragment of tcdB with primers B1C/ B2N and computationally digested these DNA fragments with AccI and HincII. The resulting RFLP patterns were interpreted following the instructions specified in the most recent toxinotyping scheme 12 .

Experiments with cell lines and animals.
To determine the virulence and pathogenic potential of HMX-149, HMX-152, and HSJD-312 we intoxicated HeLa cells with culture supernatants and fed hamsters with spore suspensions. To determine the type of cytopathic effect (CPE) elicited by the strains and their cytotoxicity (CPE 50 ), we intoxicated confluent HeLa cell monolayers with decimal dilutions of cell-free supernatants that were derived from 24 h cultures in TYT broth and monitored the cells by light microscopy. As controls, we used the non-toxigenic strain ATCC ® 700057 and a NAP1/RT027 strain from our collection. To determine the lethal activity of the Clade C-I strains, 10 000 spores resuspended in Dulbecco's modified Eagle medium (Sigma) were fed to groups of 5 Golden Syrian hamsters (150 to 180 g) that received 30 mg/kg clindamycin on day -2 through the oral route. Hamsters were monitored at 12 h intervals for signs of C. difficile infection or death. On days 1, 6, and 12, fecal pellets and intestinal contents of dead and surviving animals were processed for C. difficile isolation, and the resulting isolates were typed to confirm the identity of the inoculated strain. A NAP1 strain was tested in parallel TcdB detection. Proteins contained in 2 ml of 24 h cultures in TYT broth (3% Bacto tryptose, 2% yeast extract, and 0.1% thioglycolate, pH 6.8) were concentrated using hydroxylated silica particles (StrataClean, Agilent) and thereafter separated by electrophoresis in 7.5% SDS-polyacrylamide gels. These gels were stained with SYPRO ® Ruby (ThermoFischer Scientific) or used for electrotransfer of proteins to PVDF membranes (Macherey-Nagel) for Western Blotting with an anti-TcdB monoclonal antibody (2CV, tgcBIOMICS). For signal detection, we used a goat anti-mouse IgG-horseradish peroxidase conjugate (Invitrogen) and the Lumi-Light Plus Western Blotting substrate (Roche).
Rho glycosylation. Confluent HeLa cells grown with DMEM supplemented with 5% fetal bovine serum were intoxicated with cell-free supernatants from 24 h cultures in TYT. When a CPE was observed, the cells were lysed with 2% SDS and 20 µg of their proteins were resolved SDS-PAGE in 10% gels. The resulting gels were electro-transferred to PVDF membranes (Macherey-Nagel) that were probed with an anti-Rho monoclonal antibody that fails to recognize the glycosylated form of this small GTPase (ab54835, Abcam). Unintoxicated HeLa cells, as well as cells intoxicated with supernatant from a NAP1/027 strain, were assayed as controls.
TcdB common origin test. To test the hypothesis of a common origin for the Clade C-I TcdBs and TcdB R20291 we followed a Bayesian approach that compared the topological hypothesis of common origin versus the null hypothesis of different origin, which implies convergent evolution for the common phenotype. To rule out the possibility of recombination events affecting the analysis, the hypothesis testing was performed in a sliding window approach over the nucleotide multiple sequence alignment of tcdB with 150 and 75 nucleotides as window length and step, respectively. In each window, the likelihood of a cluster containing HMX152, HSJD312, HMX149, and R20291, was compared against the likelihood of no clustering. Both likelihoods were estimated with the Stepping Stone method 32,33 in MrBayes v3.2.6 34 , similar to the recombination analysis. Differences in the loglikelihood >5 were considered as significant evidence in favor of one or the other hypothesis. The hypothesis of positive selection was assessed for all aligned codon sites with MrBayes v3.2.6 by estimating the rate d N /d S = ω parameter. The chain was run for 200,000 states, sampling every 100 states and with the average standard deviation of split frequencies <0.01, assuming Nielsen and Yang (NY98) model of codon substitution 35 . 3D modeling of TcdB. Tridimensional structural models for the combined repetitive oligopeptides (CROPs) domains of TcdB HMX-149 , TcdB HMX-152 , and TcdB HSJD-312 were generated with the protein structure homology-modeling server SWISS-MODEL (https://swissmodel.expasy.org) using the structure PDBID 4np4 as template. Models were visualized and colored with PyMol (The PyMOL Molecular Graphics System, ver 1.3).
Antibiotic-susceptibility profiles. We determined minimum inhibitory concentrations for clindamycin, vancomycin, rifampicin, and metronidazole using BAK plates and E-tests (bioMérieux) according to the manufacturer's recommendations. For susceptibility categorization, we used the resistance breakpoints recommended by the CLSI in document M100-S27 36 .

Data Availability
Illumina sequencing data and stats are available at https://microbesng.uk/portal/projects/149D9096-03DF-4C6B-BF5F-2119D1AE7B68. The plasmid sequence of HSJD-312 was deposited in Genbank under the accession number MG973074. Other datasets generated and analyzed during this study are available from the corresponding author on reasonable request.