Figure 1: | Scientific Reports

Figure 1

From: Ultra-diluted Toxicodendron pubescens attenuates pro-inflammatory cytokines and ROS- mediated neuropathic pain in rats

Figure 1

Effect of RT on LPS mediated ROS, SOD and catalase activity in U-87 cells. (A) MTT cell viability assay after RT exposure in LPS-pre-treated U-87 cells. ***p < 0.001. (B) Biochemical detection of SOD, and (C) catalase following treatment with RT in LPS pre-treated U-87 cells. (D) LPS pre-treated (500 ng/ml for 20 min) cells were treated with various concentrations of RT prior to estimation of ROS. Graphical representation of ROS positive population analyzed by DCFH-DA staining using flow cytometer (E–K) were the graphical representation of cell count in Control, Control + LPS, RT (1 × 10−8, 1 × 10−12, 1 × 10−24 and 1 × 10−30) and H2O2 (positive control) treated U-87 cells, respectively. Data represents mean ± S.E.M. of 3 independent experiments. **p < 0.01 and ***p < 0.001 as compared to LPS treated cells.