Figure 5 | Scientific Reports

Figure 5

From: Histone H3K9 demethylase JMJD2B induces hepatic steatosis through upregulation of PPARγ2

Figure 5

Adenovirus-mediated JMJD2B overexpression stimulated hepatic steatosis in vivo. C57BL/6 mice (8 weeks old) were injected with adenovirus Ad-GFP or Ad-JMJD2B. After injection, Ad-injected mice were fed a HFD for 2 weeks. (A) JMJD2B expression was determined by qPCR (B) Immunostaining of JMJD2B (scale bar = 50 μm). Representative photographs are shown. JMJD2B positive cells were counted in ten random areas at 400× magnification and analyzed by using image J. Quantification of immunostaining assay is represented as percentage of JMJD2B positive cells. (C) Hepatic triglyceride (TG) levels were measured by a TG assay kit. (D) Liver morphology, ORO and H&E staining (scale bar = 100 μm). Representative images are presented. (E) Serum total TG levels. (F) Serum total cholesterol levels. Data represent means ± SEM from 5 mice. *p < 0.05 vs. Ad-GFP-infected mice.