Figure 4 | Scientific Reports

Figure 4

From: Histone H3K9 demethylase JMJD2B induces hepatic steatosis through upregulation of PPARγ2

Figure 4

JMJD2B lessens the enrichment of H3K9me2 and H3K9me3 on PPARγ2 promoters and increases the binding of PPARγ2 to PPREs on promoters of CD36 and PLIN2. HepG2 cells were infected with adenovirus Ad-GFP or Ad-JMJD2B. (A) The recruitment of JMJD2B to the PPARγ2 promoter was analyzed by ChIP-qPCR. (B) The enrichment of H3K9me2 and H3K9me3 on promoters of PPARγ2 was analyzed by ChIP-qPCR. (C) The recruitment of PPARγ2 to PPRE on promoters of CD36 and PLIN2 was assessed by ChIP-qPCR. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05, **p < 0.01 vs. Ad-GFP.

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