Figure 3 | Scientific Reports

Figure 3

From: Histone H3K9 demethylase JMJD2B induces hepatic steatosis through upregulation of PPARγ2

Figure 3

Knockdown of JMJD2B reduces the intracellular TG level and represses PPARγ2 expression in HepG2 cells. HepG2 cells in 6-well plates were transfected with scramble RNA or JMJD2B siRNA. (A) The levels of JMJD2B, H3K9me, H3K9me2, and H3K9me3 were measured by western blotting. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S3A. (B) Intracellular TG levels were measured by a TG assay kit. (C) PPARγ2 expression was assessed by qPCR and western blotting. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S3B. (D,E) The expression of PPARγ2 steatosis target genes was evaluated by qPCR. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05 vs. scramble RNA.

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