Figure 2 | Scientific Reports

Figure 2

From: Histone H3K9 demethylase JMJD2B induces hepatic steatosis through upregulation of PPARγ2

Figure 2

Overexpression of JMJD2B increases intracellular TG levels and stimulates PPARγ2 expression in HepG2 cells. HepG2 cells in 6-well plates were infected with Ad-GFP or Ad-JMJD2B. (A) The levels of JMJD2B, H3K9me, H3K9me2, and H3K9me3 were measured by western blotting. The full-length western blots corresponding to truncated blots are provided in Supplementary Figure S2A. (B) Intracellular TG levels were measured by TG assay kit. (C) PPARγ2 expression was assessed by qPCR and western blotting. The full-length western blots corresponding to truncated blots are presented in Supplementary Figure S2B. (D,E) The expression of PPARγ2 steatosis target genes was evaluated by qPCR. Data represent means ± SEM of three independent experiments performed in triplicate. *p < 0.05, **p < 0.01 vs. Ad-GFP.