Trajectories of vesicle motion in fibroblasts. Two examples of raw data for the vesicle positions are shown. The temporal mean squared displacement (tMSD) analysis was used to identify directed and non-directed motion. (A) A trajectory exhibiting short bursts of directed motion (v ~ 500 nm/s) followed by a long period of non-directed motion (~25 s). (B) Another trajectory showing directed motion (v ~ 700 nm/s) and non-directed motion (~8 s). The maximum possible trajectory duration in this study is 30 s due to acquisition length.