Directed differentiation of jm-iPSCs (J5F1) into neural cells (a). Schematic design of neural differentiation of jm-iPSCs. (b) Neurospheres of jm-iPSCs (J5F1) after 1-week floating culture. Scale bar; 100 µm. (c) Neuronal differentiation of neurospheres after 2-week adherent culture. Scale bar; 200 µm. (d) Immunofluorescence analyses of neural stem cell (NESTIN, PAX6) and neuron markers (TUJ1, MAP2, DREBRIN) in 2-week neuronal differentiation culture of neurospheres. Nuclei were counterstained with DAPI. Scale bar; 100 µm. (e) RT-PCR analysis of neural marker genes. β-ACTIN was examined as an internal control, and water was used as a negative control. NS, neurospheres after 2-week neuronal differentiation culture. Full-length gels are presented in Supplementary Figure S2.