Differentiation potency of jm-iPSCs (J5F1) into three germ layers (a). Schematic design of embryoid body (EB)-mediated differentiation of jm-iPSCs. (b) jm-iPSC (J5F1)-derived EBs after 2-week floating culture. Scale bar; 200 µm. (c) Outgrowth of jm-iPSC (J5F1)-derived EBs at 3-week adherent culture. Representative images of differentiated cells are shown. Scale bar; 200 µm. (d) Immunofluorescence analyses of ectoderm (TUJ1), mesoderm (α-SMA, VIMENTIN), endoderm markers (SOX17, AFP) in the EB outgrowth. Nuclei were counterstained with DAPI. Scale bar; 100 µm. (e) RT-PCR analysis of differentiation marker genes in the floating EBs. β-ACTIN was examined as an internal control, and water was used as a negative control. Full-length gels are presented in Supplementary Figure S2.