Transcriptomic differences between freshly isolated and cultured cells revealed by scRNA-Seq. (a,b) Workflow of scRNA-Seq of CPCs using 10X Genomics (a) or Smart-Seq2 technology (b). Datasets for Smart-Seq2 analyses were derived from open access transcriptome repositories for both fresh or cultured samples (scRNA-Seq datasets, blue cylinder; see supplemental table for detail information) or samples prepared in-house from cultured CPCs (represented as dish). (c,d) t-SNE plots show freshly isolated cells cluster separately from cultured cells using 10X Genomics dataset (1,615 fresh cells and 850 cultured cells; c) or Smart-seq. 2 dataset (576 fresh cells and 550 cultured cells; d). Arrow indicates cultured CPCs in (b,d). CPC, cardiac progenitor cell; BM.MSC, bone marrow-derived mesenchymal stromal cell; MEF, mouse embryonic fibroblast; ESC, embryonic stem cell; iN, induced neuronal cell; HL-1, atrial cardiomyocyte cell line; Fusion, Fusion cell of BM-MSC and HL-1; AV macrophage, atrioventricular node macrophage; E9, E11.5, E14.5, embryo 9, 11.5, 14.5 days, respectively; P7, postnatal 7 days; HSC, hematopoietic stem cell; Two-cell, embryonic cells at two-cell stage; Four-cell, embryonic cells at four-cell stage.