Figure 6 | Scientific Reports

Figure 6

From: Engineering of Primary Human B cells with CRISPR/Cas9 Targeted Nuclease

Figure 6

B cells can be engineered using homologous recombination with rAAV6 donor and CRISPR/Cas9. (A) A schematic design of the DNA donor template for a splice acceptor-EGFP system targeting the AAVS1 site by HR used in this study. (B) Percentage of EGFP+ cells represent integration frequency following transfection with rAAV6 containing a splice acceptor-EGFP system at the indicated MOIs (n = 3). (C) PCR amplification using a combination of EGFP and AAVS1 primers of samples engineered with RNPs targeting AAVS1 and exposed to rAAV6 containing a splice acceptor-EGFP system at the indicated MOIs, as well as no gRNA (represented by ‘–’) and H2O controls.

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