Figure 5 | Scientific Reports

Figure 5

From: Engineering of Primary Human B cells with CRISPR/Cas9 Targeted Nuclease

Figure 5

Simultaneous application of RNPs targeting MYC and IGH creates a t(8; 14) Burkitt Lymphoma translocation. (A) Diagram of relative positions of MYC on Chromosome 8 (left diagram), the IGH locus on Chromosome 14 (center diagram) and the t(8; 14) translocation (right diagram) associated with Burkitt Lymphoma. Arrows indicate primer direction and triangles indicate gRNA target sites. (B) Indel pattern and efficiency of RNPs targeting MYC (top panel) and IGH (bottom panel). (C) PCR amplification using MYC (left panel), IGH locus (middle panel), or a combination of MYC/IGH locus primers (right panel) of samples engineered with RNPs targeting MYC, the IGH locus, or a combination of MYC and IGH locus, as well as no gRNA (represented by ‘–’) and H2O controls.

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